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Objective@#To estimate the prevalence and the risk factors of preoperative coronary angiography (CAG) confirmed coronary stenosis in patients with degenerative valvular heart disease.@*Methods@#A total of 491 patients who underwent screening CAG before valvular surgery due to degenerative valvular heart disease were enrolled from January 2011 to September 2014 in our hospital, and clinical data were analyzed. According to CAG results, patients were divided into positive CAG result (PCAG) group or negative CAG (NCAG) group. Positive CAG result was defined as stenosis ≥50% of the diameter of the left main coronary artery or stenosis ≥70% of the diameter of left anterior descending, left circumflex artery, and right coronary artery.Risk factors of positive CAG result were analyzed by multivariable logistic regression analysis, and Bootstrap method was used to verify the results.@*Results@#There were 47(9.57%)degenerative valvular heart disease patients with PCAG. Patients were older ((68.0±7.6)years vs.(62.6±7.1)years, P<0.001) and the prevalence of typical angina was significantly higher (14.89%(7/47)vs. 2.03%(9/444), P<0.001)in PCAG group than in NCAG group. Multivariable logistic regression analysis showed that age (OR=1.118, 95%CI 1.067-1.172, P<0.001), typical angina (OR=8.970, 95%CI 2.963-27.154, P<0.001), and serum concentration of apolipoprotein B (OR=20.311, 95%CI 4.774-86.416, P<0.001) were the independent risk factors of PCAG in degenerative valvular heart disease patients. Bootstrap method revealed satisfactory repeatability of multivariable logistic regression analysis results (age: OR=1.118, 95%CI 1.068-1.178, P=0.001; typical angina: OR=8.970, 95%CI 2.338-35.891, P=0.001; serum concentration of apolipoprotein B: OR=20.311, 95%CI 4.639-91.977, P=0.001).@*Conclusions@#A low prevalence of PCAG before valvular surgery is observed in degenerative valvular heart disease patients in this patient cohort. Age, typical angina, and serum concentration of apolipoprotein B are independent risk factors of PCAG in this patient cohort.
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Objective@#To explore the plasma chemokines expressions and related clinical implication in patients with Stanford type A aortic dissection (AD).@*Methods@#We retrospectively analyzed the data of 65 patients with Stanford type A aortic dissection, hypertensive patients and 11 healthy subjects admitted in our department from October 2013 to December 2014, they were divided into four groups: NH-CON group (11 healthy subjects), H-AD group (29 AD patients with hypertension), NH-AD group (21 AD patients without hypertension), and H-CON group (14 hypertension patients). Four plasma samples from AD patients and 4 plasma samples from healthy subjects were collected randomly with random numbers table, and the levels of different chemokines were examined by protein array analysis. Then, plasma levels of chemokines including macrophage inflammatory protein 1β(MIP-1β), epithelial neutrophil activating peptide 78(ENA-78), interleukin 16(IL-16), interferon inducible protein 10(IP-10) and FMS-like tyrosine kinase 3(Flt-3) ligand were analyzed by luminex. Pearson analysis was used to determine the correlations between the chemokines and serum C reactive protein (CRP) levels.@*Results@#Plasma levels of MIP-1β(34.0(29.3, 47.2) ng/L vs. 51.0(28.2, 80.7) ng/L, P<0.05) and ENA-78(110.5(59.1, 161.4) ng/L vs. 475.7(299.3, 837.3) ng/L, P<0.05) were significantly lower in H-AD group, while plasma IL-16 level was significantly higher in H-AD group(54.7(16.3, 187.8) ng/L vs. 17.5(11.9, 20.8) ng/L, P<0.05) than in H-CON group. Plasma levels of MIP-1β(48.3(26.4, 62.1) ng/L, P<0.05) were significantly lower in H-AD patients than in NH-AD patients. Plasma level of ENA-78 was significantly lower in NH-AD group than in NH-CON group (95.0(58.0, 155.0) ng/L vs. 257.7(85.2, 397.8) ng/L, P<0.05). The levels of IP-10 and Flt-3 ligand were similar among the 4 groups (all P>0.05). Pearson analysis showed that there were no correlation between MIP-1β(r2=0.01, P>0.05), ENA-78(r2=0.02, P>0.05), IL-16(r2=0.02, P>0.05), IP-10(r2=0.00, P>0.05), Flt-3 ligand(r2=0.02, P>0.05) and CRP levels in patients with Stanford type A aortic dissection.@*Conclusions@#Lower plasma levels of MIP-1β and ENA-78 and higher plasma levels of IL-16 may associate with the occurrence and development of type A aortic dissection, but their concentrations are not correlated with serum CRP levels. There is no significant change on plasma levels of IP-10 and Flt-3 in the Stanford type A aortic dissection patients.
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Objective To explore the plasma expressions of monocyte chemotaxis proteins(MCPs) in patients with type A aortic dissection and their clinical significance.Methods 51 patients with type A aortic dissection were enrolled,in which 29 combined with hypertension.Fourteen hypertension patients and 1 1 hcalthy subjects were enrolled as control.Plasma samples were collected and we examined the levels of MCP-1,MCP-2 and MCP-4 by enzyme linked immunosorbent assay (ELISA).Results Plasma levels of MCP-1 and MCP-2 significantly decreased in patients with type A aortic dissection compared to healthy subjects(P ＜0.001),while MCP-4 had no change.Type A aortic dissection patients with or without hypertension both showed significant decreased plasma MCP-1 and MCP-2 levels compared to hypertension patients(P ＜0.001).There was no change of MCP-4 among different groups.Furthermore,Spearman correlation analysis showed that there was no correlation between serum CRP levels and plasma MCP-1,MCP-2 concentrations.Conclusion Plasma MCP-1 and MCP-2 may participate in the pathogenesis of type A aortic dissection,and their concentrations were not correlated with hypertension or serum CRP levels.The detailed mechanism needs further observations.
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Objective To study the effects of the Yinlingtong capsules on intracellular triglyceride,fatty acid oxidation levels and CPT-1/ACC-β in HHL-5 cells.Methods After treated HHL-5 cells with different doses Yinlingtong capsules,intracellular triglyceride content and the fatty acid oxidation level were detected by the kits.The CPT-1β and ACC-β mRNA levels were detected by Q-PCR.Results Yinlingtong capsules could reduce triglyceride content (compared with the control group,triglyceride content of0.5,1 and 2 mg/ml dose group were reduced by 9.5％,14.3％ and 19.4％,respectively),enhance the level of fatty acid oxidation by a dose-response relationship(compared with the control group,the level of fatty acid oxidation of 0.5,1and 2 mg/ml dose group were increased 4.3％,6.9％ and 11.2％,respectively),but could not affect the CPT-1βand ACC-β mRNA levels.Conclusion Yinlingtong capsules reduced the accumulation of fat in HHL-5 hepatocytes,and the specific impact mechanism needs to be further explored.